To analyze systemic inflammatory mediators, plasma from mice was analyzed by using the ProcartaPlex Immunoassay (ThermoFisher, Waltham, MA, USA) for granulocyte-colony stimulating factor (G-CSF), interleukin (IL)-6, keratinocyte chemoattractant (KC), IL-10, tumor necrosis factor (TNF), CXCL10 and monocyte chemoattractant protein-1 (MCP-1). All procedures were performed according to the manufacturer’s instructions. Some plasma parameters have been published as control samples in a previous study regarding cardiac inflammation after trauma (43 (link)).
Haffner-Luntzer M., Weber B., Morioka K., Lackner I., Fischer V., Bahney C., Ignatius A., Kalbitz M., Marcucio R, & Miclau T. (2023). Altered early immune response after fracture and traumatic brain injury. Frontiers in Immunology, 14, 1074207.
Other organizations :
Orthopaedic Trauma Association, University of California, San Francisco, University Hospital Frankfurt, Goethe University Frankfurt, Neurological Surgery, Steadman Philippon Research Institute
Concentration of granulocyte-colony stimulating factor (G-CSF)
Concentration of interleukin (IL)-6
Concentration of keratinocyte chemoattractant (KC)
Concentration of IL-10
Concentration of tumor necrosis factor (TNF)
Concentration of CXCL10
Concentration of monocyte chemoattractant protein-1 (MCP-1)
control variables
Plasma samples from mice
controls
Positive controls: None mentioned
Negative controls: None mentioned
The plasma parameters have been published as control samples in a previous study regarding cardiac inflammation after trauma
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