Flow cytometric analyses were performed as described (48 (link)). The following Abs were used: TLR4/MD-2 Complex APC (Thermo Fisher Scientific, 17–9924-82), F4/80 Antigen PE (Thermo Fisher Scientific, 12–4801-80), F4/80 Antigen PE Cy7 (Thermo Fisher Scientific, 25–4801-82), CD11b PE Cy7 (Biolegend, 101215), CD11b eFluor 450 (Thermo Fisher Scientific, 48–0112-82), CD11b APC (Thermo Fisher Scientific, 17–0112-82), CD45 PerCP Cy5.5 (Thermo Fisher Scientific, 45–0451-82), CD45 APC (Thermo Fisher Scientific, 17–0451-82), Ly6G APC Cy7 (Tonbo Biosciences, 25–1276), CD19 PE Cy7 (Thermo Fisher Scientific, 25–0193-82), CD4 eFluor 450 (Thermo Fisher Scientific, 48–0042-82), TCRβ (Thermo Fisher Scientific, 12–5961-83), CD8 Alexa Fluor 488 (Thermo Fisher Scientific, 53–0081-82), CD11c PerCP Cy5.5(Thermo Fisher Scientific, 45–0114-82), CD115 APC (Thermo Fisher Scientific, 17–1152-82), Fc Block (Thermo Fisher Scientific, 14–9161-71) and a Zombie Aqua Fixable Viability kit (Biolegend, 423101).
Protocol detail
Comprehensive Immune Cell Profiling
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Corresponding Organization :
Other organizations : University of Virginia
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Variable analysis
independent variables
- TLR4/MD-2 Complex APC
- F4/80 Antigen PE
- F4/80 Antigen PE Cy7
- CD11b PE Cy7
- CD11b eFluor 450
- CD11b APC
- CD45 PerCP Cy5.5
- CD45 APC
- Ly6G APC Cy7
- CD19 PE Cy7
- CD4 eFluor 450
- TCRβ
- CD8 Alexa Fluor 488
- CD11c PerCP Cy5.5
- CD115 APC
- Fc Block
- Zombie Aqua Fixable Viability kit
- Not explicitly mentioned
- Zombie Aqua Fixable Viability kit
- Positive control: Not mentioned
- Negative control: Not mentioned
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