Real-time quantitative PCR was performed on tongue as described, normalized to GAPDH (Hernández-Santos et al., 2013 (link)). Primers were from SuperArray (QIAGEN).
Protocol detail
Quantitative PCR Analysis of Tongue
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Corresponding Organization :
Other organizations : University of Pittsburgh, Children's Hospital of Pittsburgh, University of Pennsylvania, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Center for Rheumatology
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Variable analysis
independent variables
- Not explicitly mentioned
- Expression levels of unspecified genes
- GAPDH expression levels (used for normalization)
- Positive control: Not specified
- Negative control: Not specified
Annotations
This protocol is too short. Unable to provide accurate annotations
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