A total of 65 female Landrace non-heart beating donor pigs were pancreatectomized in order to study the vascular and ductal anatomy of the pancreas. The age of the animals was between 6 and 24 months. The mean weight was 452 ± 99 lb, with a range between 248 and 680 lb. All experiments were conducted according to the rules and regulations of the Institutional Animal Care and Use Committee of the University of Minnesota. Animals were heparinized, sacrificed by sodium pentobarbital overdose, and then, following the cessation of heart rhythm completely bled out and eviscerated. All of the internal organs were placed onto a procurement table. The pancreas was then removed by en bloc viscerectomy to study the pancreatic lobes and general anatomy. The vasculature was studied in situ by dissecting and identifying all the vessels that supply the pancreas, allowing for the investigation of perfusion-based preservation techniques. The pancreatic vascular supply and ductal drainage systems were studied following organ procurement bythe infusion of colored 0.9% NaCl saline.