Cadmium chloride (CdCl2), 3-mercaptoundecanoic acid (MPA), sodium citrate dihydrate sodium borohydride (Na2BH4), l-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC), Triton X-100, 2′,7′-dichlorofluorescein (H2DCF), sodium phosphate monohydrate, AlamarBlue and melittin were obtained from Sigma-Aldrich. Sodium hydroxide (NaOH) was obtained from Acros Organic. Sodium tellurite was obtained from Alfa Aeser. Tris buffer was obtained from AppliChem. Aqua (sterile water) was obtained from BBraun. Mueller Hinton Broth (MHB) was obtained from BD Difco. EDTA-blood was either drawn fresh every day of assay from an approved volunteer or purchased from Innovative Research, Inc. (Michigan, U.S.). E. coli (Strain ATCC25922) cells were purchased from the Leibniz-Institute DSMZ. Poly(oxanorbornenes) (PONs) were synthesized and characterized as reported previously (see SI for details).21 (link),23 (link) An Ultrathin LED Light Panel (Neutral White) was purchased from Environmental Lights, San Diego, CA.
Protocol detail
Nanoparticle Synthesis and Antibacterial Assay
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Cadmium chloride
Triton X-100
AlamarBlue
melittin
Sodium hydroxide (NaOH)
Sodium tellurite
Tris buffer
Acid
Alamarblue
Assay
Blood
Borohydride
Cadmium chloride
Carbodiimide
Cells
E coli
Edta
Lights
Melittin
Poly
Pons
Sodium citrate dihydrate
Sodium hydroxide
Sodium phosphate
Sodium tellurite
Sterile
Strain
Tris buffer
Triton x 100
Volunteer
Corresponding Organization : University of Maryland, Baltimore County
Other organizations : University of Freiburg
Top 5 similar protocols
Variable analysis
independent variables
- Cadmium chloride (CdCl2)
- 3-mercaptoundecanoic acid (MPA)
- Sodium citrate dihydrate
- Sodium borohydride (Na2BH4)
- L-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC)
- Triton X-100
- 2′,7′-dichlorofluorescein (H2DCF)
- Sodium phosphate monohydrate
- AlamarBlue
- Melittin
- Sodium hydroxide (NaOH)
- Sodium tellurite
- Tris buffer
- Aqua (sterile water)
- Mueller Hinton Broth (MHB)
- EDTA-blood
- E. coli (Strain ATCC25922) cells
- Poly(oxanorbornenes) (PONs)
- Not explicitly mentioned
- Not explicitly mentioned
- EDTA-blood from an approved volunteer
- Not explicitly mentioned
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