Western Blotting and Immunoprecipitation Assays

Western blot assays were performed as previously described^{37 (link)}. The primary antibodies used in our study were as follows: PEAK1 (Santa Cruz Biotechnology, USA); PEAK1/NKF3, E-cadherin, N-cadherin, MMP2, and MMP9 (Abcam, UK); p-AKT, total AKT, p-ERK1/2, total ERK1/2, p-JNK and total JNK (Invitrogen, USA); p-Rac1, total Rac1, p-JAK1, total JAK1, p-JAK2, total JAK2, Twist1, Twist2, Snail1, Snail2, Zeb1, and Zeb2 (OriGene, USA); and GAPDH (Immunology Consultants Laboratory, USA). Immunoprecipitation was performed exactly as previously described^{39 (link)}.

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Ding C., Tang W., Fan X., Wang X., Wu H., Xu H., Xu W., Gao W, & Wu G. (2018). Overexpression of PEAK1 contributes to epithelial–mesenchymal transition and tumor metastasis in lung cancer through modulating ERK1/2 and JAK2 signaling. Cell Death & Disease, 9(8), 802.

Publication 2018

E-cadherin N-cadherin MMP9 p-AKT total AKT p-ERK1/2 total ERK1/2 Snail1

Antibodies E cadherin Erk1 Gapdh Immunoprecipitation Jak1 Jak2 Mmp2 Mmp9 N cadherin Twist1 Western blot assays

Corresponding Organization : Southeast University

Other organizations : Zhongda Hospital Southeast University

Top 5 similar protocols

Variable analysis

independent variables

PEAK1
PEAK1/NKF3
E-cadherin
N-cadherin
P-AKT
Total AKT
P-ERK1/2
Total ERK1/2
P-JNK
Total JNK
P-Rac1
Total Rac1
P-JAK1
Total JAK1
P-JAK2
Total JAK2
Twist1
Twist2
Snail1
Snail2

dependent variables

Protein levels or phosphorylation status of the variables listed under independent variables

control variables

GAPDH

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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