SEM Imaging of Cell Morphology

For SEM, 10⁵ cells were fixed on microscope slides for 1 h in Karnovski solution (2% glutaraldehyde, 4% paraformaldehyde, 1 mM CaCl₂ in 0.1 M sodium cacodylate buffer), and post-fixed with 1% osmium tetroxide, for 1 h. The cells were dehydrated and then subjected to the critical point in the CPD 010 (Critical Point Dryer), plated with gold in the apparatus SCD 030 (Balzers Union, Liechtenstein). Images were taken under a JEOL JSM 6360–LV Scanning Electron Microscope (Jeol USA Inc., Peabody, MA, USA) [63 (link)]. 3 independent experiments were performed in duplicates.

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Maciel R.A., Cunha R.S., Busato V., Franco C.R., Gregório P.C., Dolenga C.J., Nakao L.S., Massy Z.A., Boullier A., Pecoits-Filho R, & Stinghen A.E. (2018). Uremia Impacts VE-Cadherin and ZO-1 Expression in Human Endothelial Cell-to-Cell Junctions. Toxins, 10(10), 404.

Publication 2018

JSM 6360–LV Scanning Electron Microscope

Buffer Cacodylate Cells Glutaraldehyde Gold Microscope Osmium tetroxide Paraformaldehyde Scanning electron microscope Sodium

Corresponding Organization :

Other organizations : Universidade Federal do Paraná, Inserm, Centre de recherche en Epidémiologie et Santé des Populations, Université de Versailles Saint-Quentin-en-Yvelines, Université Paris-Saclay, Assistance Publique – Hôpitaux de Paris, Université de Picardie Jules Verne, Pontifícia Universidade Católica do Paraná

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Variable analysis

independent variables

Fixation of 10^5 cells on microscope slides for 1 h in Karnovski solution (2% glutaraldehyde, 4% paraformaldehyde, 1 mM CaCl2 in 0.1 M sodium cacodylate buffer)
Post-fixation with 1% osmium tetroxide for 1 h
Dehydration of cells
Critical point drying in CPD 010 (Critical Point Dryer)
Gold plating in the apparatus SCD 030 (Balzers Union, Liechtenstein)

dependent variables

Images taken under a JEOL JSM 6360–LV Scanning Electron Microscope (Jeol USA Inc., Peabody, MA, USA)

control variables

3 independent experiments performed in duplicates

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