Modulating Immune Responses in Skin Transplant

For neutrophil depletion experiments, 200 μg of a rat anti–mouse Ly6G Ab (clone NIMP-R14) or irrelevant control rat Ab was injected twice i.p. into C57Bl/6 mice 24 h before and, at day 3 post-ear skin transplantation as previously described (33 (link)). Ketotifen fumarate (Sigma-Aldrich), a histamine H1-receptor antagonist, or DMSO solvent control was injected i.p. into C57Bl/6 mice at 32 mg/kg in 0.2 mL PBS 12h prior transplantation and then every day for 6 days (34 (link)). Cromolyn Sodium Salt (Sigma-Aldrich; 100 mg/kg) in 0.2 mL PBS was injected sc. 48 h, 24 h and 30 min before transplantation and then every day for 6 days to block mast cell degranulation (35 (link)).