MPTP-induced Parkinson's Disease Model

The animals belonging to the two main groups (both non-MPTP and MPTP) were divided into four subgroups according to the treatment: (i) control (untreated); (ii) NAC; (iii) HA-1077; (iv) NAC+HA-1077. NAC treated groups received a dose of 100 mg/Kg (i.p.) of NAC (Sigma Aldrich) half an hour after each injection of MPTP (NAC and MPTP+NAC and MPTP+NAC+HA-1077), following the protocol described by Pan et al. (2009) (link). A dose of 40 mg/Kg (i.p.) of HA-1077 (Sigma-Aldrich) half an hour before the first MPTP injection were administrated to HA-1077, MPTP+HA-1077, and MPTP+NAC+HA-1077 groups (Figure 1), following the protocol described by Barcia et al. (2012) (link).