Whole Genome Sequencing of Narrow-Leaf Lupin

Genome sequencing of Lupinus angustifolius was performed by the whole genome shotgun (WGS) approach [55] (link). Seeds of the single-seed-descent derived line of Tanjil were sown in the glasshouse. Three weeks after sowing, fully expanded leaves were harvested for DNA extraction. DNA was randomly sheared by nebulization, end-repaired with T4 DNA polymerase, and size selected by gel electrophoresis on 1% low-melting-point agarose. Two sequencing libraries of insert-size 500 bp and 800 bp were constructed according to the Illumina Inc. manufacturer instructions. The Pair-end sequencing of the sequencing libraries was performed on a HiSeq2000 platform. Genome sequence assembly was performed with the software program SOAPdenovo [31] , [32] (link) with a K-mer of 17. The scaffold sequences of the draft assembly from the whole genome shotgun sequencing dataset have been deposited at Genbank (Submission number “SUB139069; BioPreoject number “PRJNA179231”; website address: http://www.ncbi.nlm.nih.gov/bioproject?term=PRJNA179231).
Lupin genome annotation was performed by a homology search against the gene database of Arabidopsis (TAIR9, http://www.arabidopsis.org/) and Glycine max (Version 4.0, ftp://ftp.jgi-psf.org/pub/JGI_data/phyto-zome/v4.0/Gmax) with NCBI blast toolkit.