The ‘mice_of’ dataset was used to evaluate performance for tracking mice under optimal imaging conditions (high contrast) and with variable numbers of animals. The dataset consisted of videos from C57BL/6J male (n = 17) and female (n = 20) mice acquired from Jackson Laboratory (RRID:IMSR_JAX:000664, Jackson Laboratory). Groups of four and five mice were formed from same-sex littermates, and groups of two same-sex mice were picked randomly from different litters and interacted with each other in the open field for the first time. During video recording, mice moved freely in a 45.7 × 45.7-cm open-field arena with a clear acrylic floor. Videos were captured from below with infrared illumination using a Point Grey Blackfly S camera at a resolution of 1.97 pixels per mm at 80 FPS. Experimental procedures were approved by the Princeton University Institutional Animal Care and Use Committee and conducted in accordance with the National Institutes of Health guidelines for the humane care and use of laboratory animals. Mice used in this study had at least 1 week of acclimation to the Princeton Neuroscience Institute vivarium in group cages with food and water ad libitum under a reversed 12-h–12-h dark–light cycle (light, 19:30–07:30) and were habituated in the dark test room for at least 30 min before experimental procedures were performed. For this dataset, we labeled 1,000 frames (2,950 instances) with a skeleton consisting of 11 nodes: nose, neck, ‘L_ear’, ‘R_ear’, ‘L_Fr_paw’, ‘R_Fr_paw’, ‘tail_base’, ‘L_Hi_paw’, ‘R_Hi_paw’, ‘tail_mid’ and ‘tail_end’; and ten edges: neck to ‘L_Fr_paw’, neck to ‘R_Fr_paw’, ‘tail_base’ to ‘R_Hi_paw’, ‘tail_base’ to ‘L_Hi_paw’, ‘tail_base’ to ‘tail_mid’, ‘tail_mid’ to ‘tail_end’, neck to nose, neck to ‘R_ear’, neck to ‘L_ear’ and ‘tail_base’ to neck. Labels were randomly split into 800 training, 100 validation and 100 test frames.
Pereira T.D., Tabris N., Matsliah A., Turner D.M., Li J., Ravindranath S., Papadoyannis E.S., Normand E., Deutsch D.S., Wang Z.Y., McKenzie-Smith G.C., Mitelut C.C., Castro M.D., D’Uva J., Kislin M., Sanes D.H., Kocher S.D., Wang S.S., Falkner A.L., Shaevitz J.W, & Murthy M. (2022). SLEAP: A deep learning system for multi-animal pose tracking. Nature Methods, 19(4), 486-495.
Publication 2022
A a 1AcclimationAnimalsBlackflyFemaleFoodFramesInfrared illuminationInstitutional animal care and use committeeLaboratory animalsLightLittersMaleMiceNeckNoseSkeletonTail
Tracking performance of mice under optimal imaging conditions (high contrast) and with variable numbers of animals
control variables
Mice used were C57BL/6J male (n = 17) and female (n = 20) mice acquired from Jackson Laboratory (RRID:IMSR_JAX:000664, Jackson Laboratory)
Groups of four and five mice were formed from same-sex littermates, and groups of two same-sex mice were picked randomly from different litters and interacted with each other in the open field for the first time
During video recording, mice moved freely in a 45.7 × 45.7-cm open-field arena with a clear acrylic floor
Videos were captured from below with infrared illumination using a Point Grey Blackfly S camera at a resolution of 1.97 pixels per mm at 80 FPS
Experimental procedures were approved by the Princeton University Institutional Animal Care and Use Committee and conducted in accordance with the National Institutes of Health guidelines for the humane care and use of laboratory animals
Mice used in this study had at least 1 week of acclimation to the Princeton Neuroscience Institute vivarium in group cages with food and water ad libitum under a reversed 12-h–12-h dark–light cycle (light, 19:30–07:30) and were habituated in the dark test room for at least 30 min before experimental procedures were performed
positive controls
Not explicitly mentioned
negative controls
Not explicitly mentioned
Annotations
Based on most similar protocols
Lighting conditions should be controlled and consistent across experiments. Protocols 2 and 3 specify the use of dim lighting (25-32 lux) in the open field arena, which may help reduce stress and improve behavioral tracking (Protocol 2, Protocol 3).
Habituation of mice to the experimental setup is crucial for minimizing stress and obtaining reliable behavioral data. Protocols 2 and 5 describe extensive habituation procedures, such as acclimating mice to the vivarium, test room, and experimental apparatus over a period of 1-2 weeks prior to data collection (Protocol 2, Protocol 5).
Synchronization of behavioral recording and other experimental modalities (e.g., brain imaging, laser illumination) is important for accurate data integration. Protocol 2 describes the use of a data acquisition board and custom software to achieve this synchronization (Protocol 2).
The number of mice and their housing conditions (e.g., group vs. individual) can impact social behaviors and should be carefully considered. Protocols 1, 2, and 5 provide details on the number of mice used and their housing conditions prior to experiments (Protocol 1, Protocol 2, Protocol 5).
Cleaning the experimental arena between trials is important to prevent olfactory cues from influencing the behavior of subsequent mice. Protocols 2, 4, and 5 specify the use of multiple washes with purified water and ethanol to clean the arena (Protocol 2, Protocol 4, Protocol 5).
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As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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