Immunohistochemical Analysis of Colonic Tissue

Colonic frozen sections (4 μm thick) were stained with anti-Ki67 (Abcam, Cambridge, MA, USA), rabbit anti-CHI3L1 (Affinity Bioreagent, Golden, CO), rat anti-F4/80 (Serotec, Raleigh, NC, USA), -CD4 (eBioscience, San Diego, CA, USA), -CD11b (BD Pharmingen, San Jose, CA, USA), or -cytokeratin 8, TROMA–1 (Developmental Studies Hybridoma Bank at the University of Iowa (Iowa City, IA) antibodies, as well as normal rabbit Ig using the avidin-biotin-complex system (Vector laboratories) as previously described [12 (link)].

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Low D., DeGruttola A.K., Poltrak A., Mizoguchi A., Mino-Kenudson M, & Mizoguchi E. (2015). High Endogenous Expression of Chitinase 3-Like 1 and Excessive Epithelial Proliferation with Colonic Tumor Formation in MOLF/EiJ Mice. PLoS ONE, 10(10), e0139149.

Publication 2015

anti-Ki67 rat anti-F4/80 -CD11b TROMA–1 avidin-biotin-complex system

Antibodies Avidin Biotin Cd11b Colonic Cytokeratin 8 Frozen sections Hybridoma Rabbit Vector

Corresponding Organization : Kurume University

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Variable analysis

independent variables

Anti-Ki67 antibody
Rabbit anti-CHI3L1 antibody
Rat anti-F4/80 antibody
Anti-CD4 antibody
Anti-CD11b antibody
Anti-cytokeratin 8, TROMA–1 antibody

dependent variables

Expression of Ki67, CHI3L1, F4/80, CD4, CD11b, and cytokeratin 8 in colonic frozen sections

control variables

Colonic frozen sections (4 μm thick)

controls

Normal rabbit Ig (negative control)

Annotations

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