Immunofluorescence staining was performed as previously described (41 (link)). Primary Abs were rabbit anti-Smad1 (D59D7) and rabbit anti-p38 MAPK (Cell Signaling Technology).
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Protocol detail
Immunofluorescence Staining of Smad1 and p38
Corresponding Organization : Berlin-Brandenburger Centrum für Regenerative Therapien
Other organizations : Vlaams Instituut voor Biotechnologie, KU Leuven, Zuse Institute Berlin, Freie Universität Berlin
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Variable analysis
independent variables
- Primary Abs: rabbit anti-Smad1 (D59D7) and rabbit anti-p38 MAPK (Cell Signaling Technology)
- Unspecified
- Unspecified
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
This protocol is too short. Unable to provide accurate annotations
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