Neonatal Lung Inflammation Model

Neonatal C57BL/6J (wild type), Klrk1-/- (B6.Cg-Klrk1^tm1Dhr/J, Nkg2d-/-) and Tcrd-/- (B6.129P2-Tcrd^tm1Mom/J) mice were purchased from Jackson Laboratories (Bar Harbor, ME). Wild type and knock out mice were inoculated with 3μg/10μl of LPS from E. coli O26:B6 (Sigma-Aldrich, St. Louis, MO), or endotoxin-free PBS (Sigma-Aldrich), intranasally on day of life (DOL) 3, 5, 7, and 10 as described (28 (link)). Selected mice were injected intraperitoneally with 0.5 mg/kg of anti-IL17a antibody (clone TC11-18H10, BioLegend, San Diego, CA) or an IgG control (Rat IgG control, BioXCell, Lebanon, NH) on DOL 3, 4, 5, 6, 7, 10 and 12. Some mice were injected intraperitoneally with anti-NKG2D antibody (Clone HMG2D) or IgG control (10mg/kg) (BioXCell) 1 hr prior to each LPS treatment.

Free full text: Click here

Cui T.X., Brady A.E., Zhang Y.J., Anderson C, & Popova A.P. (2023). IL-17a-producing γδT cells and NKG2D signaling mediate bacterial endotoxin-induced neonatal lung injury: implications for bronchopulmonary dysplasia. Frontiers in Immunology, 14, 1156842.

Publication 2023

C57BL/6J LPS from E. coli O26:B6 endotoxin-free PBS Rat IgG control IgG control

Anti antibody Clone E coli Endotoxin Il17a Klrk1 Knock out mice Mice Neonatal Tcrd

Corresponding Organization : University of Michigan–Ann Arbor

Top 5 similar protocols

Variable analysis

independent variables

Genotype of mice (wild type, Klrk1-/-, Tcrd-/-)
Treatment with LPS or endotoxin-free PBS
Administration of anti-IL17a antibody or IgG control
Administration of anti-NKG2D antibody or IgG control

dependent variables

Not explicitly mentioned

control variables

Day of life (DOL) on which mice were inoculated (3, 5, 7, 10)
Dose of LPS (3μg/10μl)
Dose of anti-IL17a antibody (0.5 mg/kg)
Dose of anti-NKG2D antibody (10 mg/kg)

controls

Positive control: Wild type mice inoculated with LPS
Negative control: Wild type and knockout mice inoculated with endotoxin-free PBS
IgG control for anti-IL17a and anti-NKG2D antibody treatments

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!