Histological Evaluation of Mouse Colon

Mouse colon tissues were fixed in 4% paraformaldehyde (Servicebio, Wuhan, China) for 12 hours and embedded. Sections were processed and stained with hematoxylin and eosin (H&E) or Alcian blue (AB) for pathological evaluation. The histological score was evaluated according to the inflammatory infiltration and mucosal damage as described previously (27 (link)). Representative images were captured using a fluorescence inversion microscope (Nikon, Shanghai, China).

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Wang P., Liu D., Zhou Z., Liu F., Shen Y., You Q., Lu S, & Wu J. (2023). The role of protein arginine deiminase 4-dependent neutrophil extracellular traps formation in ulcerative colitis. Frontiers in Immunology, 14, 1144976.

Publication 2023

paraformaldehyde fluorescence inversion microscope

Alcian blue Colon Eosin Fluorescence microscope Inflammatory Inversion Mouse Mucosal Paraformaldehyde Tissues

Corresponding Organization : Tulane University

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Variable analysis

independent variables

Paraformaldehyde fixation duration (12 hours)

dependent variables

Histological score (based on inflammatory infiltration and mucosal damage)
Microscopic images

control variables

Mouse colon tissues
Paraformaldehyde concentration (4%)
Hematoxylin and eosin (H&E) staining
Alcian blue (AB) staining

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