Antioxidant Enzyme Assays in Leaf Tissue
Corresponding Organization : Cairo University
Other organizations : Indian Agricultural Research Institute
Protocol cited in 6 other protocols
Variable analysis
- Leaf tissue homogenate prepared in 50 mM potassium phosphate buffer (pH 7.0), containing 10% (w/v) polyvinylpyrrolidone (PVP), 0.25% (v/v) Triton X-100, 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 mM ASC
- Superoxide dismutase (SOD) activity
- Peroxidase (POX) activity
- Catalase (CAT) activity
- Glutathione peroxidase (GPX) activity
- Ascorbate peroxidase (APX) activity
- Glutathione reductase (GR) activity
- Glutathione-S-transferase (GST) activity
- Monodehydroascorbate reductase (MDHAR) activity
- Dehydroascorbate reductase (DHAR) activity
- Leaf tissue mass (100 mg fresh weight)
- Volume of buffer used for homogenization (1 ml)
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