Multilineage Differentiation Potential of MSCs

Multilineage differentiation potential of AML-MSCs and HD-MSCs was identified as we reported [12 (link), 14 (link), 17 (link)]. In brief, 5×10⁴ cells were seeded into 12-well plate wells in MSC culture medium for 2–3 days. After reaching 60–80% confluence, the indicated AML-MSCs and HD-MSCs were cultured in adipogenic-, osteogenic-, and chondrogenic-differentiation medium (Stem Cell Technologies) according to the manufacturer’s instructions, respectively. After 3 weeks’ induction, the MSC-derived adipocytes, osteoblasts, and chondrocytes were identified by Oil Red O staining, Alizarin Red S staining, and Alcian Blue staining, respectively.

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Zhang L., Chi Y., Wei Y., Zhang W., Wang F., Zhang L., Zou L., Song B., Zhao X, & Han Z. (2021). Bone marrow-derived mesenchymal stem/stromal cells in patients with acute myeloid leukemia reveal transcriptome alterations and deficiency in cellular vitality. Stem Cell Research & Therapy, 12, 365.

Publication 2021

chondrogenic-differentiation medium

Adipocytes Adipogenic Alcian blue Alizarin red s Cells Chondrocytes Chondrogenic Osteoblasts Osteogenic Stem cell

Corresponding Organization : First Affiliated Hospital of Soochow University

Other organizations : Second Hospital of Hebei Medical University, Hebei Medical University

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Variable analysis

independent variables

Cell type (AML-MSCs and HD-MSCs)

dependent variables

Adipogenic differentiation (identified by Oil Red O staining)
Osteogenic differentiation (identified by Alizarin Red S staining)
Chondrogenic differentiation (identified by Alcian Blue staining)

control variables

Seeding density (5×10^4 cells per well)
Culture medium (MSC culture medium)
Confluence level (60-80%) before induction
Induction duration (3 weeks)

controls

Positive controls: None specified
Negative controls: None specified

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