Focal Cerebral Ischemia in Rats

Adult male Sprague–Dawley rats were anesthetized with chloral hydrate (400 mg/kg, i.p.). The right MCA was ligated and bilateral common carotids (CCAs) were clamped for 90-min to generate focal ischemia in the cerebral cortex12 (link), 13 (link), 14 (link). Core body temperature was maintained at 37 °C.

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Luo Y., Kuo C.C., Shen H., Chou J., Greig N.H., Hoffer B.J, & Wang Y. (2009). Delayed treatment with a p53 inhibitor enhances recovery in stroke brain. Annals of neurology, 65(5), 520-530.

Publication 2008

Adult Body temperature Carotids Cerebral ischemia Chloral hydrate Male Sprague dawley rats

Corresponding Organization :

Other organizations : National Institute on Drug Abuse, National Institutes of Health, Institute on Aging

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Protocol cited in 11 other protocols

Variable analysis

independent variables

Right MCA ligation
Bilateral common carotids (CCAs) clamping for 90 minutes

dependent variables

Focal ischemia in the cerebral cortex

control variables

Core body temperature maintained at 37 °C
Anesthesia with chloral hydrate (400 mg/kg, i.p.)

controls

No positive controls mentioned
No negative controls mentioned

Annotations

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