Pseudomonas sp. 1502IPR-01 Genome Assembly

The complete genome sequence of Pseudomonas sp. 1502IPR-01 was assembled using both the PacBio and Illumina reads. The original image data was transferred into sequence data via base calling, defined as raw data, and saved as a FASTQ file. Those FASTQ files contain read sequences, and quality information is included. A statistic of quality information was applied for quality trimming, by which the low-quality data can be removed to form clean data. The reads were then assembled into a contig using a hierarchical genome assembly process (HGAP) and canu, a software that performs scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation. The last circular step was checked and finished manually, generating a complete genome with seamless chromosomes and plasmids. Finally, error correction of the PacBio assembly results was performed using the Illumina reads using Pilon. All of the above analyses were performed using I-Sanger Cloud Platform (www.i-sanger.com) from Shanghai Majorbio.