Immunohistochemistry Staining of Xenografts

For immunohistochemistry, formalin fixed, paraffin embedded 4 μm sections of the xenografts were stained using a Ventana Benchmark Immunostainer (Ventana Medical Systems, Inc, Arizona, USA) previously [45 (link)]. Immunohistochemistry images were taken using Axioskop 2 microscope, captured using a Nikon DXM1200C digital camera and processed using NIS-Elements F3.0 software. Images were scored independently by four reviewers blind to the molecular data as previously described [46 (link)].

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Abubaker K., Luwor R.B., Zhu H., McNally O., Quinn M.A., Burns C.J., Thompson E.W., Findlay J.K, & Ahmed N. (2014). Inhibition of the JAK2/STAT3 pathway in ovarian cancer results in the loss of cancer stem cell-like characteristics and a reduced tumor burden. BMC Cancer, 14, 317.

Publication 2014

Ventana Benchmark Immunostainer DXM1200C

Blind Camera Embedded paraffin Formalin Immunohistochemistry Microscope Xenografts

Corresponding Organization :

Other organizations : Royal Women's Hospital, St Vincent's Hospital, University of Melbourne, Royal Melbourne Hospital, Walter and Eliza Hall Institute of Medical Research

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Variable analysis

independent variables

Immunohistochemistry protocol

dependent variables

Immunohistochemistry images
Immunohistochemistry image scores

control variables

Formalin fixed, paraffin embedded 4 μm sections of the xenografts
Ventana Benchmark Immunostainer
Axioskop 2 microscope
Nikon DXM1200C digital camera
NIS-Elements F3.0 software
Four independent reviewers blind to the molecular data

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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