Imiquimod-Induced Skin Inflammation

Shaved mouse dorsal skin was treated daily for 5 consecutive days with 30 mg Aldara cream containing 5% Imiquimod (IMQ, Meda AB). On day 5, full-thickness skin biopsies of the treated area were collected with a 8 mm biopsy puncher; skin was either snap frozen in liquid N₂ for RNA extraction, fixed in neutral buffered formalin (Sigma) for histopathology analysis, or digested as described below to achieve single-cell suspensions. In some experiments, wild-type C57BL/6 mice received vehicle (olive oil) or 100 μg/kg FICZ (Enzo) intraperitoneally on the day before starting the IMQ treatment and then daily until the day of analysis.

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Di Meglio P., Duarte J.H., Ahlfors H., Owens N.D., Li Y., Villanova F., Tosi I., Hirota K., Nestle F.O., Mrowietz U., Gilchrist M.J, & Stockinger B. (2014). Activation of the Aryl Hydrocarbon Receptor Dampens the Severity of Inflammatory Skin Conditions. Immunity, 40(6), 989-1001.

Publication 2014

neutral buffered formalin

Aldara Biopsy C57bl mice Cell Ficz Formalin Frozen Mouse Olive oil Skin

Corresponding Organization :

Other organizations : The Francis Crick Institute, King's College London, Clinical Research Center Kiel

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Variable analysis

independent variables

Treatment with 30 mg Aldara cream containing 5% Imiquimod (IMQ) for 5 consecutive days
Administration of 100 μg/kg FICZ intraperitoneally on the day before starting the IMQ treatment and then daily until the day of analysis

dependent variables

Gene expression (through RNA extraction)
Histopathological changes (through tissue fixation and analysis)
Single-cell suspension (through tissue digestion)

control variables

Shaved mouse dorsal skin
Vehicle (olive oil) administration in some experiments

controls

Positive control: FICZ treatment
Negative control: Vehicle (olive oil) treatment

Annotations

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