Fixed tissues were processed in Leica TP 1020 Tissue Processor and paraffin
embedded using HistoCore Arcadia (Leica Biosystems). Five micrometers tissue
sections were cut using a microtome (HistoCore AUTOCUT; Leica Biosystems) and
mounted on Polysine™ adhesion microscopic slides (Epredia). Hematoxylin and
Eosin staining was performed following the protocol of Suzuki et al.16 (link) Briefly,
slides containing tissue sections were deparaffinized followed by hydration in a
series of ethanol dilutions. Harris hematoxylin solution (HHS32, Sigma Aldrich,
UK) was added to stain the nuclei (purple) followed by washing to remove excess
stain. Eosin Y solution (HT110216, Sigma Aldrich, UK) was then added to stain
the cytoplasm, collagen and connective tissue (pink). After washing and
dehydration, the slides were mounted and imaging done using a light microscope
Motic BA210 series (Motic, Xiamen, China) integrated ColorVu camera.
embedded using HistoCore Arcadia (Leica Biosystems). Five micrometers tissue
sections were cut using a microtome (HistoCore AUTOCUT; Leica Biosystems) and
mounted on Polysine™ adhesion microscopic slides (Epredia). Hematoxylin and
Eosin staining was performed following the protocol of Suzuki et al.16 (link) Briefly,
slides containing tissue sections were deparaffinized followed by hydration in a
series of ethanol dilutions. Harris hematoxylin solution (HHS32, Sigma Aldrich,
UK) was added to stain the nuclei (purple) followed by washing to remove excess
stain. Eosin Y solution (HT110216, Sigma Aldrich, UK) was then added to stain
the cytoplasm, collagen and connective tissue (pink). After washing and
dehydration, the slides were mounted and imaging done using a light microscope
Motic BA210 series (Motic, Xiamen, China) integrated ColorVu camera.
