Differentiation of Naive CD4+ T Cells

Differentiation of CD4⁺ T cells was performed according to our previous study^{17 (link)}. In brief, splenic naïve CD4⁺ T cells were sorted from the indicated mice (8 to 12 weeks of age) by an EasySep™ mouse naïve CD4⁺ T cell isolation kit (STEMCELL Technologies, 19765). Th17, Th1 and Th2 polarizations were then conducted following the instructions of the CellXVivo mouse Th17 cell differentiation kit (R&D Systems, CDK017), the CellXVivo mouse Th1 cell differentiation kit (R&D Systems, CDK018) and the CellXVivo mouse Th2 cell differentiation kit (R&D Systems, CDK019), respectively.

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Wang X., Han C., Yang D., Zhou J., Dong H., Wei Z., Xu S., Xu C., Zhang Y., Sun Y., Ni B., Guo S., Zhang J., Zhao T., Chen X., Luo J., Wu Y, & Tian Y. (2024). STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells. Communications Biology, 7, 10.

Publication 2024

EasySep™ mouse naïve CD4+ T cell isolation kit CellXVivo mouse Th17 cell differentiation kit CellXVivo mouse Th1 cell differentiation kit CellXVivo mouse Th2 cell differentiation kit

Corresponding Organization :

Other organizations : Qingdao University, Army Medical University, Southwest Hospital, Xinqiao Hospital, Chinese PLA General Hospital

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Variable analysis

independent variables

Mouse strain (indicated mice, 8 to 12 weeks of age)

dependent variables

Th17, Th1 and Th2 cell differentiation

control variables

Splenic naïve CD4+ T cells sorted using EasySep™ mouse naïve CD4+ T cell isolation kit

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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