Proteins were tryptic digested before passing through the C18 column packed with 1.9umAqua C18 material (Phenomenex). Peptides eluted from the LC column were directly electrosprayed into a Q-Exactive plus mass spectrometer (ThermoFisher, San Jose, CA). MS scan functions and HPLC solvent gradients were controlled by the Xcalibur data system (ThermoFisher). All raw files were processed using Maxquant software (version 1.5.3.30) (Beer et al. 2017 (link)) for feature detection, database searching, and protein/peptide quantification. MS/MS spectra were searched against the UniProtKB/Swiss-Prot human database (containing 20,386 reviewed sequences). The minimum peptide length was seven amino acids, and maximum peptide mass was 4600 Da. The allowed missed cleavages for each peptide was two. The second peptide search was activated to identify coeluting and cofragmented peptides from one MS/MS spectrum. Both peptides and proteins were filtered with a maximum FDR of 0.01. LFQ calculations were performed separately in each parameter group. Both unique and razor peptides were selected for protein quantification. Other unmentioned parameters were the default settings of the Maxquant software (Beer et al. 2017 (link)).