PD-L1 Immunohistochemistry in NEN

FFPE tissue sections were stained with the Dako PD-L1 22C3 pharmDx kit (Agilent Technologies, Santa Clara, CA, USA). In previous studies, PD-L1 immunohistochemistry (IHC) was scored by either combined positive score (CPS) or tumor proportion score (TPS), since there is no recommended PD-L1 evaluation criteria for ICI treatment in NEN heretofore [36 (link),37 (link),38 (link)]. CPS represents the percentage of the number of PD-L1 positive cells, including tumor cells, lymphocytes, and macrophages, to the total number of viable tumor cells, whereas TPS only notes the proportion of PD-L1 positive tumor cells [14 (link)]. In this study, two board-certificated pathologists (S.C. and S.K.) independently interpreted the PD-L1 22C3 IHC slides and then made a consensus CPS.

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Cho H.G., Cho S.I., Choi S., Jung W., Shin J., Park G., Moon J., Ma M., Song H., Mostafavi M., Kang M., Pereira S., Paeng K., Yoo D., Ock C.Y, & Kim S. (2022). Artificial Intelligence-Powered Whole-Slide Image Analyzer Reveals a Distinctive Distribution of Tumor-Infiltrating Lymphocytes in Neuroendocrine Neoplasms. Diagnostics, 12(10), 2340.

Publication 2022

Dako PD-L1 22C3 pharmDx kit

Immunohistochemistry Lymphocytes Macrophages Pathologists Pd l1 Tissue Tumor

Corresponding Organization : Ajou University

Other organizations : State University of New York, SUNY Downstate Health Sciences University, Samsung Medical Center, Sungkyunkwan University, Korea University

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Variable analysis

independent variables

PD-L1 immunohistochemistry (IHC) scoring method (CPS or TPS)

dependent variables

Percentage of PD-L1 positive cells (including tumor cells, lymphocytes, and macrophages) out of total viable tumor cells (CPS)
Proportion of PD-L1 positive tumor cells (TPS)

control variables

Tissue samples (FFPE tissue sections)
Staining protocol (Dako PD-L1 22C3 pharmDx kit)
Evaluation of PD-L1 IHC slides by two board-certified pathologists

controls

No positive or negative controls were explicitly mentioned in the input text.

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