Quantifying ZEB2 Expression in Aortic Tissue

Immunohistochemistry was performed on de-paraffinized aortic tissue sections treated with DIVA solution (Biocare Medical, Concord, CA, USA), as described previously [5 (link)]. The expression of ZEB2 was quantified in n = 10 BAV-ND patients, and n = 11 TAV-ND patients, using mouse monoclonal anti-ZEB2 (Santa Cruz, Heidelberg, Germany, sc-271984). Quantification of per cent area positive staining was performed using Image-Pro® Premier version 9.1 Software (Media Cybernetics®, Silver Spring, MD, USA).

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Maleki S., Cottrill K.A., Poujade F.‐., Bhattachariya A., Bergman O., Gådin J.R., Simon N., Lundströmer K., Franco‐Cereceda A., Björck H.M., Chan S.Y, & Eriksson P. (2018). The mir‐200 family regulates key pathogenic events in ascending aortas of individuals with bicuspid aortic valves. Journal of Internal Medicine, 285(1), 102-114.

Publication 2018

DIVA solution anti-ZEB2 Image-Pro® Premier version 9.1 Software

Aortic Immunohistochemistry Mouse Patients Silver Tissue

Corresponding Organization : Karolinska University Hospital

Other organizations : University of Pittsburgh Medical Center

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Variable analysis

independent variables

Presence of bicuspid aortic valve (BAV-ND) vs. tricuspid aortic valve (TAV-ND)

dependent variables

Expression of ZEB2 protein

control variables

De-paraffinized aortic tissue sections
Treatment with DIVA solution (Biocare Medical, Concord, CA, USA)
Use of mouse monoclonal anti-ZEB2 antibody (Santa Cruz, Heidelberg, Germany, sc-271984)
Quantification of percent area positive staining using Image-Pro® Premier version 9.1 Software (Media Cybernetics®, Silver Spring, MD, USA)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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