Microdevice Fabrication Using Polycarbonate

The design and toolpaths for the microdevice were created in Autodesk Fusion 360 (Autodesk, San Rafael, CA, USA) and were custom-milled (Shapeoko, Carbide 3D, Torrance, CA, USA) out of polycarbonate [26 (link)]. The final device was manufactured by pouring polydimethylsiloxane (PDMS) mixed in a 10:1 base to a curing agent ratio (Sylgard 184 elastomer kit; Dow Corning, Midland, MI, USA). PDMS was cured at 80 °C for 3 h, peeled off, and cut into individual devices. Channel inlets and outlets with 1.5 mm diameter were punched at both ends of microfluidic channels. The PDMS devices were permanently bound to the detergent-cleaned glass coverslips after plasma treatment for 50 s (Model PDC-001-HP, Harrick Plasma, Ithaca, NY, USA) for the subsequent lipid bilayer formation and substrate modification.