Comparative Analysis of Aconitum Chloroplast Genomes

The cp genomes of 6 reported Aconitum species and 1 exogenous species were loaded from the NCBI website, which are, A. flavum (MW839582.1), A. pendulum (MW839578.1), A. brachypodum (NC_041579.1), A. vilmorinianum (MH063436.1), A. kusnezoffii (MK253471.1), A. carmichaelii (KY407560.1), and D. yunnanense (MW246158.1). CGView software was used to evaluate the cp genome structures of the eight plants [39 (link)]. Mauve v2.3.1 was used to analyze at the homology and covariance of cp sequences [40 (link)]. For broad comparison of homologous gene sequences from different plants, the MAFFT v7.310 (automatic mode) [41 (link)] was employed. Nucleotide diversity (PI) values for each gene were calculated using DNAsp v5.0 [42 (link)]. The IR, SSC, and LSC region boundary information was visualized using the SVG package in Perl. MAFFT v7.310 software was used to compare gene sequences, and Ka/Ks Calculator v2.0 software was utilized to calculate the Ka/Ks values of the genes.

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Yanfei N., Tai S., Chunhua W., Jia D, & Fazhong Y. (2023). Complete chloroplast genome sequences of the medicinal plant Aconitum transsectum (Ranunculaceae): comparative analysis and phylogenetic relationships. BMC Genomics, 24, 90.

Publication 2023

Aconitum Genes Genome Nucleotide Plants Plants structures

Corresponding Organization :

Other organizations : Southwest Forestry University, Xinjiang Institute of Materia Medica, State Forestry and Grassland Administration

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Chloroplast genome structures of the 8 plant species
Homology and covariance of chloroplast sequences
Nucleotide diversity (PI) values for each gene
Ka/Ks values of the genes

control variables

None explicitly mentioned

controls

No positive or negative controls were specified by the authors.

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