Evaluating Osteoblast Viability via CCK-8 Assay

Cell Counting Kit-8 (CCK8) method is used to detect the cell viability of cultured human hFOB1. 19 osteoblasts. The experimental operation process is carried out according to the technical methods provided in the manual. The simple experimental process is as follows. After transfection, hFOBl. 19 osteoblasts are cultured for 24–27 h and used for CCK-8 analysis of cell activity. Cells in each group are collected at three different time points, namely, 24, 48 and 72 h. First, pipettes are used to gently remove old media. Then, according to the ratio of 100:1, the fresh complete culture medium is diluted with CCK-8 reagent. Finally, the cell culture plate $\mathrm{n}\left(\mathrm{j}\right)$ is put back into the incubator to continue to culture for about 1 h, and then detected by the microplate reader for statistical analysis of the difference ${\mathrm{c}}_{\mathrm{y}}$ in cell activity among groups. At least three biological repeats shall be set for the above analysis (Wang et al., 2019 (link)). $k_g=g_{FS}-g$
$\mathrm{n}\left(\mathrm{j}\right)=\frac{∆\mathrm{l}}{∆\mathrm{z}*\mathrm{n}}$
${\mathrm{a}}_{\mathrm{a}\mathrm{s}}=\frac{1}{\mathrm{d}\left(\mathrm{\lambda }\right)}$
${\mathrm{c}}_{\mathrm{y}}=\mathrm{h}+\frac{\mathrm{g}}{{\mathrm{P}}_{\max }\bullet {\mathrm{t}}_0}$
During the miR-206 expression level difference experiment, the femoral head tissue samples of 15 patients with femoral neck fracture who underwent total hip arthroplasty were collected as the control group.