Rat Model of Liver Fibrosis: Treatment Evaluation

After a one-week of acclimatization, rats were divided into five groups, each with six animals, according to the following scheme: Group 1: the negative control group; rats were injected intraperitoneally (ip) with saline three times per week for two successive weeks, Group 2: the positive control (TAA) group; rats were ip injected with TAA (100 mg/kg) three times per week for two successive weeks to provoke liver fibrosis [27 (link)] () with some modification based on our preliminary studies. Group 3 and 4: treatment groups; rats received orally RBO (0.2 and 0.4 mL/kg, orally) [28 (link), 29 (link)] daily for 2 weeks after 2 weeks of TAA injection. Group 5: reference group; rats received silymarin (100 mg/kg, orally) [30 (link)], daily for 2 weeks after 2 weeks of TAA injection.

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Abdel-Rahman R.F., Fayed H.M., Asaad G.F., Ogaly H.A., Hessin A.F., Salama A.A., Abd El-Rahman S.S., Arbid M.S, & Mohamed M.A. (2021). The involvement of TGF-β1 /FAK/α-SMA pathway in the antifibrotic impact of rice bran oil on thioacetamide-induced liver fibrosis in rats. PLoS ONE, 16(12), e0260130.

Publication 2021

Acclimatization Animals Liver fibrosis Rats Saline Silymarin

Corresponding Organization : National Research Centre

Other organizations : King Khalid University, Cairo University

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Variable analysis

independent variables

Type of treatment: Saline, TAA, RBO (0.2 and 0.4 mL/kg), or Silymarin (100 mg/kg)

dependent variables

Liver fibrosis

control variables

Duration of treatment (2 weeks)
Route of administration (intraperitoneal for TAA, oral for RBO and Silymarin)
Number of animals per group (6)
Acclimatization period (1 week)

controls

Negative control, Rats injected intraperitoneally with saline three times per week for two successive weeks
Positive control (TAA), Rats injected intraperitoneally with TAA (100 mg/kg) three times per week for two successive weeks to provoke liver fibrosis

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