Multiparameter Flow Cytometry Panels

The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PECY7 (SP34-2, BD), CD3-PB (SP34-2, BD), CD4-APC (L200, BD, used for measuring CD4 expression after CD4 depleting Ab treatment), CD8-PB (RPA-T8, BD), CD8-APC (RPA-T8, BD), CD8-PE (RPA-T8, BD), IFN-γ-Allophycocyanin (4S.B3, BD), IFN-γ-PE (4S.B3, BD), TNF-α-PE (MAb11, BD), TNF-α-APC (MAb11, BD), TNF-α-PB (MAb11, eBioscience), IL-17-PE (eBio64CAP17, eBioscience), IL-17-Alexa647 (eBio64CAP17, eBioscience), IL-22-biotinylated (anti-human IL-22, RD, see reference(44 (link))), Streptavidin-Pacific blue (invitrogen). IL-4-APC (8D4-8, eBioscience), Perforin-biotinylated (Pf-344, Mabtech)

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Yao S., Huang D., Chen C.Y., Halliday L., Wang R.C, & Chen Z.W. (2014). CD4+ T cells are required to contain early extrathoracic TB dissemination and sustain multi-effector functions of CD8+ T and CD3− lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2120-2132.

Publication 2014

CD49d (9F10, CD3-PB (SP34-2, CD4-APC CD8-PB (RPA-T8, CD8-APC (RPA-T8, CD8-PE RPA-T8, IFN-γ-PE (4S.B3, TNF-α-PE TNF-α-APC eBio64CAP17 Streptavidin-Pacific blue Pf-344

Alexa647 Allophycocyanin Cytokine Flow cytometry Human Ifn γ Il 17 Il 22 Intracellular Perforin Streptavidin Tnf α

Corresponding Organization :

Other organizations : University of Illinois Urbana-Champaign, University of Illinois Chicago, Illinois College

Top 5 similar protocols

Variable analysis

independent variables

CD28 (CD28.2, BD)
CD49d (9F10, BD)
CD4 depleting Ab treatment

dependent variables

CD3-PECY7 (SP34-2, BD)
CD3-PB (SP34-2, BD)
CD4-APC (L200, BD)
CD8-PB (RPA-T8, BD)
CD8-APC (RPA-T8, BD)
CD8-PE (RPA-T8, BD)
IFN-γ-Allophycocyanin (4S.B3, BD)
IFN-γ-PE (4S.B3, BD)
TNF-α-PE (MAb11, BD)
TNF-α-APC (MAb11, BD)
TNF-α-PB (MAb11, eBioscience)
IL-17-PE (eBio64CAP17, eBioscience)
IL-17-Alexa647 (eBio64CAP17, eBioscience)
IL-22-biotinylated (anti-human IL-22, RD)
IL-4-APC (8D4-8, eBioscience)
Perforin-biotinylated (Pf-344, Mabtech)

control variables

Streptavidin-Pacific blue (invitrogen)

Annotations

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