HPLC analysis: The solution was filtered with membrane filter (0.22 μm). 20 μL Solution was injected into HPLC (Waters 1525 HPLC) column Kromat Universil C18 (4.6 mm × 250 mm, 5 μm) operating at 28°C with CH3OH:H2O:HAc (25:74:1, v/v/v) carrier liquid (flow rate: 1.1 mL/min). Calibration curves of all analytes routinely yielded correlation coefficients 0.999 or better, and the detection of the compounds was carried out with a UV-detector at 280 nm.
Nitrobenzene Oxidation of Lignin
HPLC analysis: The solution was filtered with membrane filter (0.22 μm). 20 μL Solution was injected into HPLC (Waters 1525 HPLC) column Kromat Universil C18 (4.6 mm × 250 mm, 5 μm) operating at 28°C with CH3OH:H2O:HAc (25:74:1, v/v/v) carrier liquid (flow rate: 1.1 mL/min). Calibration curves of all analytes routinely yielded correlation coefficients 0.999 or better, and the detection of the compounds was carried out with a UV-detector at 280 nm.
Corresponding Organization :
Other organizations : Huazhong Agricultural University
Protocol cited in 3 other protocols
Variable analysis
- Extraction solvent (benzene-ethanol, 2:1, v/v)
- Nitrobenzene concentration (0.5 mL)
- NaOH concentration (2 M)
- Reaction temperature (170°C)
- Reaction time (3.5 h)
- Yields of p-hydroxybenzaldehyde (H), vanillin (G), and syringaldehyde (S) from nitrobenzene oxidation of cell wall residue (CWR)
- Mass of CWR (0.05 g)
- Stirring speed (20 rpm)
- HPLC column (Kromat Universil C18, 4.6 mm × 250 mm, 5 μm)
- HPLC mobile phase (CH3OH:H2O:HAc, 25:74:1, v/v/v)
- HPLC flow rate (1.1 mL/min)
- HPLC column temperature (28°C)
- HPLC detection wavelength (280 nm)
- Chromatographic internal standard (ethyl vanillin)
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