Fecal samples were resuspended in PBS at a concentration of 50 mg/mL by extensive vortexing, allowed to stand for 20 min, and centrifuged at 16,000 g for 10 min to collect supernatant as described (Haneberg et al., 1994 (link)). Each supernatant was assessed for IgA using the mouse IgA ELISA kit with suitable dilutions, according to the manufacturer’s instructions (Bethyl, Montgomery TX), and the absorbance was measured at a wavelength of 450 nm using the Dynex MRX TC Revelation microplate reader (Dynex Technologies, Chantilly VA) (Ruiz et al., 2017 (link)).
Other organizations :
NYU Langone Health, Wisconsin Institutes for Discovery, New York University, University of North Carolina at Chapel Hill, University of Minnesota, Janssen (United Kingdom), Johnson & Johnson (United Kingdom), Tufts University
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