Chromosome Spread Immunostaining Protocol

Chromosome spreads were prepared from adult Syce3testes as described^{49 (link)}.
Chromosome spreads were stained with antibodies as described^{29 (link)}. Primary antibodies were mouse
anti-SYCP3 (Abcam #ab97672, 1:500), rabbit anti-SYCP1 (Abcam #ab15090, 1:200)
and rabbit anti-RAD51 (Millipore #PC 130, 1:500). Slides were mounted using
antifade mounting medium (Vectashield, H-1000) and high precision coverslips
(Marienfeld).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Crichton J.H., Dunce J.M., Dunne O.M., Salmon L.J., Devenney P.S., Lawson J., Adams I.R, & Davies O.R. (2023). Structural maturation of SYCP1-mediated meiotic chromosome synapsis by SYCE3. Nature structural & molecular biology, 30(2), 188-199.

Publication 2023

ab97672 ab15090 PC 130 H-1000 high precision coverslips

Adult Antibodies Chromosome Rabbit

Corresponding Organization :

Other organizations : University of Edinburgh, MRC Institute of Genetics and Molecular Medicine, Newcastle University

Top 5 similar protocols

Variable analysis

independent variables

Chromosome spreads from adult Syce3 testes

dependent variables

Staining with antibodies against SYCP3, SYCP1, and RAD51

control variables

Antibody concentrations (1:500 for anti-SYCP3, 1:200 for anti-SYCP1, 1:500 for anti-RAD51)
Mounting medium (antifade mounting medium, Vectashield H-1000)
Coverslips (high precision coverslips, Marienfeld)

controls

Positive control: Staining with antibodies as described in the reference (PMID: 28708824)
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!