A portion of the bulk soil samples was used to determine the metal composition using Total Reflection X-Ray Fluorescence (TXRF) technique. Briefly, for each sample 1 g of soil was resuspended in 1 mL of distilled water and homogenized for 2 h at room temperature. After mixing, the samples were centrifuged at 11,440 g for 10 min in a Hettich Universal 32R. The soluble fraction was recovered and measured in a Bruker S2 PICOFOX.
Soil texture (sand:silt:clay), electrical conductivity, and nitrogen levels (total N, NO3−, NH4+) were provided by the Laboratorio Agroanálisis UC, Facultad de. Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, according to the methods established by the Normalization and Accreditation Commission (CNA) of the Chilean Society of Soil Science93 , as performed before62 (link).
For soil pH measurement, 2 g of soil were mixed with 5 mL milliQ water and homogenized during 2 h at RT (S1000 Gyrotwister) at 60 rpm. Afterwards, the soil was decanted during 2 h. The pH was measured in triplicate for each sample in a Thermo Scientific Orion 3 star benchtop pH meter.
Soil texture (sand:silt:clay), electrical conductivity, and nitrogen levels (total N, NO3−, NH4+) were provided by the Laboratorio Agroanálisis UC, Facultad de. Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, according to the methods established by the Normalization and Accreditation Commission (CNA) of the Chilean Society of Soil Science93 , as performed before62 (link).
For soil pH measurement, 2 g of soil were mixed with 5 mL milliQ water and homogenized during 2 h at RT (S1000 Gyrotwister) at 60 rpm. Afterwards, the soil was decanted during 2 h. The pH was measured in triplicate for each sample in a Thermo Scientific Orion 3 star benchtop pH meter.
Free full text: Click here
