The study consisted of 4 visits at the 1st, 7th, 10th and 13th day of observation (Fig 1 ).
All visits started at 9:00 AM and included medical examination, blood pressure measurement, and resting electrocardiography to exclude any contraindications to exercise test. At the first visit (day 1st) subjects underwent spirometry tests (measurement of FVC, FEV1 and FEV1/FVC ratio) and treadmill VO2 max test. Afterwards, at the three consecutive visits (day 7th, 10th, and 13th) participants performed treadmill exercise to volitional exhaustion at speed corresponding to 70% of their personal VO2 max. Pre- and immediately post-exhaustive exercise venous blood samples (15 ml) were collected into vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) with EDTA for cell free DNA extraction and blood cell count, into tubes containing gel and clot activator for blood chemistry, and into tubes with sodium oxalate and potassium fluoride for lactate determination. All exercise bouts were performed at the Academic Laboratory of Movement and Human Physical Performance “DynamoLab” of the Medical University of Lodz at ambient temperature 20–21°C and relative air humidity 50–60%. During the whole study period (13 days) volunteers did not perform any exhaustive exercise besides those related to study protocol. The study was conducted according to the Declaration of Helsinki. The protocol was reviewed and approved by The Medical University of Lodz Ethics Committee (RNN/95/14/KB), and all volunteers provided written informed consent.
All visits started at 9:00 AM and included medical examination, blood pressure measurement, and resting electrocardiography to exclude any contraindications to exercise test. At the first visit (day 1st) subjects underwent spirometry tests (measurement of FVC, FEV1 and FEV1/FVC ratio) and treadmill VO2 max test. Afterwards, at the three consecutive visits (day 7th, 10th, and 13th) participants performed treadmill exercise to volitional exhaustion at speed corresponding to 70% of their personal VO2 max. Pre- and immediately post-exhaustive exercise venous blood samples (15 ml) were collected into vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) with EDTA for cell free DNA extraction and blood cell count, into tubes containing gel and clot activator for blood chemistry, and into tubes with sodium oxalate and potassium fluoride for lactate determination. All exercise bouts were performed at the Academic Laboratory of Movement and Human Physical Performance “DynamoLab” of the Medical University of Lodz at ambient temperature 20–21°C and relative air humidity 50–60%. During the whole study period (13 days) volunteers did not perform any exhaustive exercise besides those related to study protocol. The study was conducted according to the Declaration of Helsinki. The protocol was reviewed and approved by The Medical University of Lodz Ethics Committee (RNN/95/14/KB), and all volunteers provided written informed consent.
Free full text: Click here
