U266 cells were treated with 30 μM of DDZ for 24 h, and MM1.S cells were pre-treated with 30 μM of DDZ for 3 h and treated with IL-6 (10 ng/mL) for 21 h. Late apoptotic cell death was determined with a Roche Diagnosis TUNEL assay kit, as described before [44 (link)].
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