The clinical laboratory of CRUN is situated on the same compound as CMA. Laboratory practices have been standardized using Standard Operational Procedures (SOPs) and a quality management system according to Good Clinical Laboratory Practices principles is implemented.
Upon inclusion venous blood was sampled for malaria microscopy, full blood count (Sysmex XS1000i (Sysmex Corporation, Kobe, Japan)) and blood cultures (1–3 ml pediatric culture bottle (BD BACTEC Peds PlusTM /F, (Becton Dickinson and Company, Sparks, Maryland, USA)) as previously described [11 (link)]. Malaria was diagnosed by microscopy [12 ] and, at HC Nazoanga, also by rapid diagnostic test (RDT, SD Bioline Antigene Pf (Standard Diagnostics, Hagal-Dong, Korea)).
Samples from CMA were transported to CRUN within 15 minutes after collection. For HC Nazoanga, samples were stored and transported to CRUN by car (ambient temperature for blood cultures, 2–8°C for EDTA-anticoagulated blood) at the end of each day and transport time was recorded. The quality indicators are reported in S1 Appendix.
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