Real-Time Cell Migration and Invasion Assay

The cell migration and invasion experiments were performed using the xCELLigence RTCA DP system (Roche, Mannheim, Germany) as described previously.^{23 (link), 50 (link)} Briefly, cells serum-starved for 6 h were resuspended with serum-free medium and seed into up chamber of CIM-plate 16 with or without precoated Matrigel (1:40 dilution), which the low chamber was added medium containing 5% FBS, for real-time cell analysis by using the RTCA DP Instrument. The increase of the impedance correlates with increasing numbers of migrated cells were recorded every 15 min and the cell index Slope (1/hour) for a time period/range was calculated according to RTCA Software Manual (Version 1.2).

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Zhao X., Deng R., Wang Y., Zhang H., Dou J., Li L., Du Y., Chen R., Cheng J, & Yu J. (2017). Twist1/Dnmt3a and miR186 establish a regulatory circuit that controls inflammation-associated prostate cancer progression. Oncogenesis, 6(4), e315-.

Publication 2017

xCELLigence RTCA DP system

Cell Cell migration Dilution Matrigel Rtca Serum

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University

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Variable analysis

independent variables

Presence or absence of pre-coated Matrigel in the upper chamber

dependent variables

Cell migration and invasion (as measured by the increase in impedance and the cell index slope)

control variables

Cell serum starvation for 6 hours
Serum-free medium in the upper chamber
Medium with 5% FBS in the lower chamber

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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