Characterization of Leukemic Cell Lines

Human leukemic cell lines SU-DHL-6, OCL-LY-19, SU-DHL-16 (DLBCL), and DOHH2 (FL) were obtained from Dr. Eric Hsi and Dr. Neetu Gupta (Cleveland Clinic), Nalm6, Reh (acute lymphoblastic leukemia) were purchased from the American Type Culture Collection (Manassas, VA, USA), Mec-2 from Dr. Y. Saunthararajah (Cleveland Clinic) and MO1040 (both CLL) from Dr. Riccardo Dalla-Favera (Columbia University) and used as before.^{32 (link), 33 (link), 45 (link)} All cell lines were cultured in RPMI-1640 medium supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA, USA), and antibiotic-antimycotic (Gibco, Life Technologies, Gaithersburg, MD, USA). ABT199-R cells were cultured with 5% FBS. Cell lines were routinely screened for Mycoplasma, variations in growth rates, changes in morphological characteristics, and their response to stress with Annexin V FITC-PI staining; their passage number did not exceed 20. ABT-737 and ABT-199 were obtained from AbbVie (Chicago, IL, USA); NVP-BEZ235, RAD001, and GS-1101 from Selleck Chemicals (Houston, TX, USA); and verapamil and cycloheximide from Sigma-Aldrich (St. Louis, MO, USA).