Histopathological Analysis of Lung Tissue

Histopathological analysis was performed as described before^{25 (link)}. Briefly, mice were sacrificed and lung tissues were inflated and fixed in PFA, embedded in paraffin or frozen in optimal cutting temperature compound (OCT) and sectioned for hematoxylin and eosin (H&E) staining. Immunohistochemical and immunofluorescence analyses were performed as described^{34 (link)}. Antibodies against the following proteins were used: KI-67 (Novocastra Laboratories Ltd), CDC42 (Abcam), ZO1 (33-9100, Zymed); PAR6 (sc-14405, Santa Cruz), Occludin (353197a Zymed), Phalloidin (R415 Invitrogen), CCSP (Santa Cruz), SP-C (AB3786, Chemicon), Beta Galactosidase (ab9631, Abcam), Biotinylated goat anti-rabbit secondary antibody (ZYMED company), Alexa Fluor 555 or 488 conjugated anti-mouse, rat or rabbit IgG secondary antibodies (Invitrogen).

Free full text: Click here

Zheng C., Wang Y., Yang L., Zhou S., Gao Y., Li F., Feng Y., Wang Z., Zhan L., Yan Q., Zhu X., Wong K.K., Chen Z, & Ji H. (2017). Cell Division Cycle 42 plays a Cell type-Specific role in Lung Tumorigenesis. Scientific Reports, 7, 10407.

Publication 2017

CDC42 Occludin Beta Galactosidase Biotinylated goat anti-rabbit secondary antibody

Alexa fluor 555 Anti antibody Antibodies Beta galactosidase Cdc42 Embedded paraffin Eosin Frozen Goat Hematoxylin Igg antibodies Immunofluorescence Lung Mouse Occludin Phalloidin Proteins Rabbit Tissues

Corresponding Organization :

Other organizations : Shanghai Institutes for Biological Sciences, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Yale University, Dana-Farber Cancer Institute

Top 5 similar protocols

Variable analysis

independent variables

Sacrifice of mice
Lung tissue fixation and embedding (in paraffin or OCT)
Histological sectioning and staining (H&E, immunohistochemistry, immunofluorescence)

dependent variables

Histopathological analysis of lung tissues

control variables

Described experimental procedures (as described before in references 25 and 34)

controls

Positive control: None explicitly mentioned
Negative control: None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!