Isolation and culture of human PBMC has been previously described.14 15 (link) Central memory T (Tcm) cells were enriched by negative selection of CD14, CD25, and CD45RA and positive selection of CD62L. For expansion, cells were maintained in complete X-VIVO media with 50 U/mL recombinant human IL2 (Novartis) and 0.5 ng/mL recombinant human IL15 (CellGenix).
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