Isolation and Expansion of Central Memory T Cells
Corresponding Organization : City Of Hope National Medical Center
Variable analysis
- Enrichment of central memory T (Tcm) cells by negative selection of CD14, CD25, and CD45RA and positive selection of CD62L
- Not explicitly mentioned
- Maintenance of cells in complete X-VIVO media with 50 U/mL recombinant human IL2 (Novartis) and 0.5 ng/mL recombinant human IL15 (CellGenix) for expansion
- No positive or negative controls were explicitly mentioned in the provided information
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