Laser-induced choroidal neovascularization model

The laser-induced CNV model was generated as described previously [20 (link)]. In brief, laser injury (532 nm, 180 mW, 75 μm, 100 ms, Novus Spectra; Lumenis, Tokyo, Japan) was conducted around the optic disc using a slit-lamp delivery system. Four laser spots per eye for quantification of CNV and choroidal fibrosis were performed. The formation of a subretinal bubble at the time of laser injury indicated the rupture of Bruch’s membrane. Immediately after laser injury, 1 μg of PDGFR-β neutralizing antibody (APB5, Thermo Fisher Scientific, Waltham, MA, USA) or isotype-matched IgG (Thermo Fisher Scientific) in 1 μL, was injected into the vitreous cavity of mice. An additional intravitreal injection was given to mice on day 7 for the quantification of subretinal fibrosis formation at 21 days after CNV induction.