In all experiments mice were administered the selective COMT inhibitor tolcapone (30mg/kg; TRC Inc)(25 (link),41 (link)) and/or the selective DAT blocker GBR-12909 dihydrochloride (6mg/kg, Tocris)(42 (link)). These doses are effective at altering behavior without causing motor stereotypies (see Supplementary Information ). D-amphetamine (in sulfate formulation; Tocris) was used at 4mg/kg (43 (link)), and the NET blocker atomoxetine (in hydrochloride formulation; Tocris) at 1mg/kg (44 (link)) in the voltammetry experiment only. All drugs were dissolved in 20% hydroxypropyl-beta-cyclodextrin (Acros Organics) in 0.9% saline (AquPharm), which served as a vehicle control in all experiments. All drugs were delivered by intraperitoneal injection.
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Selective Neuromodulator Effects on Mouse Behavior
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0 9 saline
Atomoxetine
Comt
D amphetamine
Drugs
Gbr 12909
Hydroxypropyl beta cyclodextrin
Intraperitoneal injection
Mice
Sulfate
Tolcapone
Corresponding Organization : Wellcome Centre for Integrative Neuroimaging
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Variable analysis
independent variables
- Tolcapone (30mg/kg)
- GBR-12909 dihydrochloride (6mg/kg)
- D-amphetamine (4mg/kg)
- Atomoxetine (1mg/kg)
- Not explicitly mentioned
- Vehicle control (20% hydroxypropyl-beta-cyclodextrin in 0.9% saline)
- Doses of drugs chosen to alter behavior without causing motor stereotypies (see Supplementary Information)
- Positive control: None mentioned
- Negative control: Vehicle control (20% hydroxypropyl-beta-cyclodextrin in 0.9% saline)
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