Biomimetic Brain Tissue Hydrogel

Brain tissue-mimicking hydrogel was prepared by interpenetrating growth-factor-reduced Matrigel matrix (Corning) and matrix metalloproteinase (MMP)-sensitive hyaluronic acid (HA) hydrogels with a volume ratio of 1:1. MMP-sensitive HA hydrogel was synthesized as described previously (Wang et al., 2019 (link)). Briefly, HA-ADH was firstly obtained using hyaluronic acid (100 mg, 0.0015 mmole, 50 kDa), ADH (2.6 g, 8.4 mmole) and 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) (0.3 g, 0.92 mmole) at pH 4.75, followed by dialysis (MWCO 6–8 kDa) in deionized water for 2–3 days and lyophilizing. Acrylated hyaluronic acid (HA-AC) was prepared by reacting the synthesized HA-ADH (100 mg, 0.0014 mmole) with N-Acryloxysuccinimide (NHS-AC) (108 mg, 0.47 mmole) in HEPES buffer overnight and dialysis in DI water for 2–3 days before lyophilizing. HA-AC was further conjugated with RGD peptides (Ac-GRGDSPCG-NH2, Genscript) overnight, dialysis in DI water for 2 days and lyophilizing. Finally, MMP-sensitive HA hydrogel (10 mg/mL) was obtained by crosslinking with MMP-degradable crosslinker (GCRDVPMSMRGGDRCG, Genscript).

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Cui X., Ma C., Vasudevaraja V., Serrano J., Tong J., Peng Y., Delorenzo M., Shen G., Frenster J., Morales R.T., Qian W., Tsirigos A., Chi A.S., Jain R., Kurz S.C., Sulman E.P., Placantonakis D.G., Snuderl M, & Chen W. (2020). Dissecting the immunosuppressive tumor microenvironments in Glioblastoma-on-a-Chip for optimized PD-1 immunotherapy. eLife, 9, e52253.

Publication 2020

Matrigel matrix GCRDVPMSMRGGDRCG

Brain Buffer Carbodiimide Dialysis Growth factor Hepes Hyaluronic acid Hydrogel Matrigel Matrix metalloproteinase Matrix metalloproteinase 1 Rgd peptides Tissue

Corresponding Organization :

Other organizations : Jinan University, New York University, NYU Langone Health

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Variable analysis

independent variables

Volume ratio of growth-factor-reduced Matrigel matrix and MMP-sensitive hyaluronic acid (HA) hydrogels

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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