Comprehensive Fatty Acid Profiling and Metabolism

Individual FAs within the same class (saturated FAs, monounsaturated FAs [MUFAs], polyunsaturated FAs [PUFAs], n-3, n-6, n-7, and n-9) were summed as follows to quantify total FA levels: total saturated FAs: lauric acid + myristic acid + pentadecanoic acid + palmitic acid + margaric acid + stearic acid + arachidic acid; total MUFAs: palmitoleic acid + heptadecanoic acid + oleic acid + gadoleic acid + erucic acid + nervonic acid; total PUFAs: ALA + stearidonic acid + 11,14,17-eicosatrienoic acid (ETE) + EPA + DPA + DHA + LA + GLA + DGLA + ARA + adrenic acid + osbond acid + 5,8,11-eicosatrienoic acid; total n-3 PUFAs: ALA + stearidonic acid + ETE + EPA + DPA + DHA; total n-6 PUFAs: LA + GLA + DGLA + ARA + adrenic acid + osbond acid; total n-7 FAs: palmitoleic acid + heptadecanoic acid; total n-9 FAs: oleic acid + gadoleic acid + erucic acid + nervonic acid + 5,8,11-eicosatrienoic acid.
Ratios of product/substrate FAs were used as in vivo activity markers for the following desaturases and elongases: stearoyl-CoA desaturase-16 (SCD16): palmitoleic acid/palmitic acid [88 (link)]; stearoyl-CoA desaturase-18 (SCD18): oleic acid/stearic acid [53 (link)]; delta-6-desaturase (D6D): GLA/LA [88 (link)]; delta-5-desaturase (D5D): ARA/DGLA [88 (link)]; elongase-6 (ELOVL6): stearic acid/palmitic acid [89 (link)]; elongase-5 (ELOVL5): DGLA/GLA [89 (link)]; elongase-2 (ELOVL2): adrenic acid/ARA [90 (link)].

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Nguyen N., Woodside D.B., Lam E., Quehenberger O., German J.B, & Shih P.A. (2024). Fatty Acids and Their Lipogenic Enzymes in Anorexia Nervosa Clinical Subtypes. International Journal of Molecular Sciences, 25(10), 5516.

Publication 2024

Corresponding Organization : University of California, San Diego

Other organizations : University Health Network, University of California, Davis

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Variable analysis

independent variables

Fatty acid types and classes (saturated FAs, monounsaturated FAs [MUFAs], polyunsaturated FAs [PUFAs], n-3, n-6, n-7, and n-9)

dependent variables

Total levels of saturated FAs, MUFAs, PUFAs, n-3 PUFAs, n-6 PUFAs, n-7 FAs, and n-9 FAs
Ratios of product/substrate FAs used as in vivo activity markers for desaturases and elongases (stearoyl-CoA desaturase-16 [SCD16], stearoyl-CoA desaturase-18 [SCD18], delta-6-desaturase [D6D], delta-5-desaturase [D5D], elongase-6 [ELOVL6], elongase-5 [ELOVL5], elongase-2 [ELOVL2])

control variables

No control variables explicitly mentioned

positive controls

No positive controls explicitly mentioned

negative controls

No negative controls explicitly mentioned

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