NMR-Based Body Composition Analysis

Body composition was assessed before tumor implantation, at randomization and 2.5 weeks after treatment via nuclear magnetic resonance using LF110 BCA-Analyzer (Bruker Corporation, Billerica, MA, USA) as described previously [72 (link)]. Briefly, at ~0700, animals were removed from their cage and weighed. Animals were then placed in a restrainer and inserted into the NMR for approximately 2 min. Fat and fat-free mass were determined by calibration with internal standards according to manufacturer’s instructions.

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Zunica E.R., Axelrod C.L., Cho E., Spielmann G., Davuluri G., Alexopoulos S.J., Beretta M., Hoehn K.L., Dantas W.S., Stadler K., King W.T., Pergola K., Irving B.A., Langohr I.M., Yang S., Hoppel C.L., Gilmore L.A, & Kirwan J.P. (2021). Breast cancer growth and proliferation is suppressed by the mitochondrial targeted furazano[3,4-b]pyrazine BAM15. Cancer & Metabolism, 9, 36.

Publication 2021

LF110 BCA-Analyzer

After treatment Animals Body composition Implantation Tumor

Corresponding Organization :

Other organizations : Pennington Biomedical Research Center, Louisiana State University, UNSW Sydney, Case Western Reserve University

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Variable analysis

independent variables

Time point of body composition assessment

dependent variables

Fat mass
Fat-free mass

control variables

Timing of measurements (~0700)
Duration of NMR scan (approximately 2 min)
Calibration with internal standards

controls

No positive or negative controls were explicitly mentioned in the provided information.

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