Biocompatibility Evaluation of Cerium-Doped Hydroxyapatite Nanoparticles

The biocompatibility of the obtained cerium-doped HAp powders with AFSC was evaluated using fluorescence microscopy with a RED CMTPX fluorophore (Thermo Fischer, Waltham, MA, USA), a cell tracker for long-term tracing of living cells. The CMTPX tracker was added in cell culture, priory treated with the synthesized nanoparticles. After 5 days, the viability and morphology of the AFSC was evaluated. The CMTPX fluorophore, at a concentration of 5 µM and incubated for 30 min, was added in the culture medium, to allow the dye penetration into the cells. Finally, the AFSC were washed with PBS and visualized by fluorescent microscopy. The photomicrographs were taken using an Olympus CKX 41 digital camera driven by CellSense Entry software (Olympus, Tokyo, Japan) [38 (link),39 (link)].