Histological Analysis of Liver, Pancreas and Adipose Tissue

Samples of the liver, pancreas, and epididymal adipose tissue were fixed with 10% formalin solution. After fixation, the specimens were dehydrated, embedded in paraffin, cut in a microtome to a thickness of 5 mm each, and stained with hematoxylin and eosin [41 (link),43 (link)]. An expert pathologist performed a blind histological analysis of the liver and pancreas and classified the samples according to a score system [45 (link),46 (link)]. Pancreas analysis followed the architecture of pancreas evaluation, according to changes in the islets of Langerhans [41 (link),43 (link),47 (link),48 (link)].
The adipocyte area of the epididymal adipose tissue was photographed by using a Leica DFC 495 digital camera system (Leica Microsystems, Wetzlar, Germany) integrated into a Leica DM 5500B microscope (Leica Microsystems, Wetzlar, Germany), with a magnification of 200x. The images were analyzed by using the Leica Application Suite software, version 4.0 (Leica Microsystems, Wetzlar, Germany), and a mean area of 100 adipocytes per sample was determined [49 (link)].

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Inada A.C., Silva G.T., da Silva L.P., Alves F.M., Filiú W.F., Asato M.A., Junior W.H., Corsino J., Figueiredo P.D., Garcez F.R., Garcez W.S., da Silva R.D., dos Santos-Eichler R.A., Guimarães R.D., Freitas K.D, & Hiane P.A. (2020). Therapeutic Effects of Morinda citrifolia Linn. (Noni) Aqueous Fruit Extract on the Glucose and Lipid Metabolism in High-Fat/High-Fructose-Fed Swiss Mice. Nutrients, 12(11), 3439.

Publication 2020

Leica DM 5500B microscope Leica Application Suite software, version 4.0

Adipocytes Adipose tissue Blind Camera Embedded paraffin Eosin Epididymal Formalin Islets of langerhans Liver Microscope Microtome Pancreas Pathologist

Corresponding Organization : Universidade Federal de Mato Grosso do Sul

Other organizations : Universidade de São Paulo

Top 5 similar protocols

Variable analysis

independent variables

Tissue samples (liver, pancreas, and epididymal adipose tissue)

dependent variables

Histological analysis and classification of liver and pancreas samples
Adipocyte area of epididymal adipose tissue

control variables

Fixation with 10% formalin solution
Dehydration, embedding in paraffin, and cutting to 5 mm thickness
Staining with hematoxylin and eosin

controls

Blind histological analysis by an expert pathologist

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