NSCLC Drug Screening Protocol from GDSC

Drug screening data were downloaded from Genomics of Drug Sensitivity in Cancer (GDSC) Project (https://www.cancerrxgene.org/downloads/bulk_download, accessed on 15 April 2021) [53 (link)]. The GDSC Project screened more than 1,000 genetically characterized human cancer cell lines with a wide range of anti-cancer therapeutic agents. Among the commonly used chemotherapeutic regimens in treating NSCLC drugs, nine were found in GDSC1, including cisplatin, docetaxel, erlotinib, etoposide, gefitinib, gemcitabine, pemetrexed, and vinorelbine; seven were found in GDSC2, including cisplatin, docetaxel, erlotinib, gefitinib, gemcitabine, paclitaxel, and vinorelbine. For each drug, cell lines were defined as resistant, sensitive, or partial response by using the mean ± 0.5 SD of the IC₅₀ values as described above.